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Winner Announced – NovaSeq Mini Grant – How would you use 3 billion reads?

We are excited to announce that scientists from CSIRO’s Synthetic Biology Future Science Platform, the Australian Institute of Marine Science with the National Sea Simulator, and the University of Melbourne are the winners of the NovaSeq Mini Grant –  “What would you do with 3 billion reads.”  The group will receive sequencing data from an Illumina NovaSeq 6000 S2 sequencing run valued at $24,300. Congratulations!

The project aims to engineer microbial symbionts to increase corals temperature resilience:

Coral reefs worldwide, including two thirds of the Great Barrier Reef, have suffered extreme bleaching events and mass mortality from high seawater temperatures over the last decades. Current efforts to reduce such climate change associated impacts might be too little to prevent severe long-term impacts on reefs. Therefore, it is essential to consider alternative mitigation strategies, such as genetic technologies to improve the thermal tolerance of corals and their symbiotic algae (Symbiodinium).

Our group is working towards engineering microbial symbionts to increase coral temperature resilience. This project is an interdisciplinary collaboration of scientists from CSIRO’s Synthetic Biology Future Science Platform, the Australian Institute of Marine Science and the University of Melbourne (Dr. Patrick Buerger, Prof. Madeleine van Oppen, Dr. John Oakeshott and Dr. Owain Edwards). We use single cell transcriptomics and gene expression profiles to investigate the genetic mechanisms underpinning the algal symbiont’s thermal tolerance. This information will be used to identify target genes and cellular pathways that are suitable for engineering increased temperature resilience into these symbiotic microalgae.

 

New Technology and a New Facility

In April of this year the Ramaciotti Centre for Genomics moved to UNSW’s new $165M Biosciences South Building and in June commissioned $2M of new genomic sequencing technology. This brings outstanding new capabilities in genomics to Australia. The Centre was formally openrd by the NSW Chief Scientists Professor Mary O’Kane read more….

Short Read Sequencing  – Illumina NovaSeq
In June the Ramaciotti Centre installed and validated an Illumina NovaSeq 6000 system.  The NovaSeq has scalable throughput and flexibility for virtually any sequencing method or genome and brings unprecedented short read sequencing capacity to Australia. The Centre now has the ability to facilitate any size or type of sequencing project using our suite of Illumina instruments. The Centre has Illumina Certified Service Provider (CSPro) status. 

Long Read Sequencing – PacBio Sequel
The Ramaciotti Centre also installed a PacBio Sequel system in June adding additional long read sequencing capacity to our portfolio. The Centre was one of the first sites in Australia to install the PacBio RSII system and since then we have completed many successful SMRT sequencing projects for researchers around Australia and overseas. The placement of the Sequel means that Ramaciotti Centre is the only site in Australia with both the RSII and Sequel systems and thus able to offer researchers the flexibility of PacBio SMRT sequencing on either platform. The Centre has PacBio Certified Service Provider (CSP) status. 

Linked Read & High Throughput Single Cell – 10x Genomics Chromium
The 10X Genomics Chromium system provides long range genomic or cell-by-cell gene expression information, thereby unlocking further utility on our existing short-read sequencers. The system supports single cell 3’ transcript profiling within complex cell populations and provides long-range information on a genome-wide scale, including variant calling, phasing and extensive characterization of genomic structure. 

Please contact us for further information on services provided on the above technology platforms. 

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