Sample Requirements

The information below serves only as a general guide on sample requirements by application. Project specific sample submission information is included in our quotes. If you cannot provide the requested amount of nucleic acid please contact us for options.

General Sample Submission Criteria

Samples should be intact i.e. not degraded.
Samples should be free of contaminants: 260:280 1.8 - 2.2 and 260:230 ratio >1.5.
RNA samples must be free of genomic DNA contamination.
DNA samples must be free of RNA contamination.
DNA concentration should be measured using the Qubit or Picogreen assay as quantification by spectrophotometric methods is inaccurate.

Application Specific Sample Submission Criteria

Genome Sequencing - Short Read

Service	Amount Requested	Concentration	Minimum Volume
PCR-free sample prep	1 ug DNA	30-60 ng/ul	30 ul
PCR-plus sample prep (Illumina DNA Prep)	500ng DNA	20-25 ng/ul	25 ul

Service

Amount Requested

Concentration

Minimum Volume

PCR-free sample prep

1 ug DNA

30-60 ng/ul

30 ul

PCR-plus sample prep

(Illumina DNA Prep)

500ng DNA

20-25 ng/ul

25 ul

RNA Sequencing - Short Read

Service	Amount Requested	Concentration	Minimum Volume
mRNA seq (Illumina Stranded mRNA Prep)	1.2 ug total RNA	30-40 ng/ul	40 ul
Total RNA seq - human, mouse, rat, bacteria (Illumina Stranded Total RNA Prep with Ribo-Zero Plus)	400 ng total RNA	20-50 ng/ul	20 ul
Total RNA seq - plants (TruSeq Stranded Total RNA with Ribo-Zero Plant)	3 ug total RNA	120-180 ng/ul	25 ul
Total RNA seq - low input (SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input)	10-20 ng total RNA	1-4 ng/ul	12 ul
Total RNA seq - rRNA depleted submission	120 ng rRNA depleted RNA	10-20 ng/ul	12 ul
small RNA* (QIAseq miRNA Library Kit)	600 ng total RNA	40-100 ng/ul	15 ul

* contact us for input requirements for serum and plasma.

Client Prepared Libraries

Refer to your quote for the amount for this service.
Library fragments up to 1kb.
Custom primers must be supplied at the time of sample submission.
One pool per run (unless prior arrangements have been made).

Exome and Panel Sequencing

We provide various capture options requiring different input amounts. Contact us for further information.

Microbiome Sequencing

15-20ul of DNA at a concentration of 5-10ng/ul.
Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
DNA of high purity (260/280, 260/230 >~1.8).
Samples must be amplifiable by PCR (please check this prior to submission).

ChIP Sequencing

5ng of the enriched material in a total volume of 12-16ul.
Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
The fragment size range of the enriched material should be 200-600bp.

Gene Expression & Transcriptome Microarrays

Service	Amount Required	Concentration	Minimum Volume
Standard assay	500 ng total RNA	50-100 ng/ul	10 ul
Low input (pico) assay	500 pg total RNA	50-100 pg/ul	10 ul

Genotyping by Microarray

50ul of intact genomic DNA at a concentration of 15 - 50 ng/ul.
Quantification must be performed by a fluorescence assay (Qubit or Picogreen).
Samples should be resuspended in nuclease free water or low EDTA TE buffer.
The 260:280 OD ratio should be 1.8 - 2.2 and the 260:230 OD ratio >1.5.
DNA should be intact as assessed by agarose gel or similar method.
Note: We can accept dried samples if plate sealing is a concern.

Karyotyping Arrays

20ul of intact genomic DNA at a concentration of 50-100 ng/ul.
Quantification must be performed by a fluorescence assay (Qubit or Picogreen).
Samples should be resuspended in nuclease free water or low EDTA TE buffer.
The 260:280 OD ratio should be 1.8 - 2.0 and the 260:230 OD ratios >1.5.
DNA should be intact as assessed by agarose gel or similar method.

Pluripotency Confirmation Arrays

10ul of intact total RNA at a concentration of 50-100 ng/ul.

Oxford Nanopore Long Read Sequencing

Contact us for further information or refer to your quote. Our Long & Linked Read Sample Submission Guide provides general information on DNA/RNA quality.