The most accurate sample submission information can be found in our quotes. The information below serves only as a general guide on sample requirements by application e.g. RNA sequencing.
General Sample Submission Criteria
- Samples should be intact i.e. not degraded.
- Samples should be free of contaminants: 260:280 1.8 - 2.2 and 260:230 ratio >1.5.
- RNA samples must be free of genomic DNA contamination.
- DNA samples must be free of RNA contamination.
- DNA concentration should be measured using the Qubit or Picogreen assay as quantification by spectrophotometric methods is inaccurate.
Application Specific Sample Submission Criteria
Genome Sequencing - Short Read
| Service | Amount Required | Concentration | Minimum Volume |
|---|---|---|---|
| TruSeq PCR-free |
4 ug DNA |
50-100 ng/ul |
60 ul |
| Nextera DNA Flex |
500ng DNA |
20 ng/ul |
25 ul |
|
Nextera XT DNA |
100ng DNA |
5 ng/ul |
20 ul |
RNA Sequencing - Short Read
| Service | Amount Required | Concentration | Minimum Volume |
|---|---|---|---|
| mRNA seq |
3 ug total RNA |
70-100 ng/ul |
40 ul |
| Total RNA seq* |
3 ug total RNA |
120-180 ng/ul |
25 ul |
|
Total RNA seq - low input |
10-20 ng total RNA |
1-2 ng/ul |
10 ul |
| Total RNA seq - rRNA depleted submission |
300 ng rRNA depleted total RNA |
20-30 ng/ul |
15 ul |
|
small RNA |
1 ug total RNA |
80-100 ng/ul |
12 ul |
* Species supported: human, mouse, rat and plant.
Client Prepared Libraries
- Refer to page 1 of your quote for the amount for this service.
- Library fragments up to 1kb.
- Custom primers must be supplied at the time of sample submission.
- One pool per run (unless prior arrangements have been made).
Exome and Panel Sequencing
We provide various capture options requiring different input amounts. Contact us for further information.
Microbiome Sequencing
- 15-20ul of DNA at a concentration of 5-10ng/ul.
- Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
- DNA of high purity (260/280, 260/230 >~1.8).
- Samples must be amplifiable by PCR (please check this prior to submission).
ChIP Sequencing
- 10ng of the enriched material in a total volume of 20ul.
- Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
- The fragment size range of the enriched material should be 200-600bp.
Gene Expression & Transcriptome Microarrays
| Service | Amount Required | Concentration | Minimum Volume |
|---|---|---|---|
| Standard assay |
500 ng total RNA |
50-100 ng/ul |
10 ul |
| Low input (pico) assay |
500 pg total RNA |
50-100 pg/ul |
10 ul |
Genotyping by Microarray
- 50ul of intact genomic DNA at a concentration of 15 - 50 ng/ul.
- Quantification must be performed by a fluorescence assay (Qubit or Picogreen).
- Samples should be resuspended in nuclease free water or low EDTA TE buffer.
- The 260:280 OD ratio should be 1.8 - 2.2 and the 260:230 OD ratio >1.5.
- DNA should be intact as assessed by agarose gel or similar method.
- Note: We can accept dried samples if plate sealing is a concern.
Karyotyping Arrays
- 20ul of intact genomic DNA at a concentration of 50-100 ng/ul.
- Quantification must be performed by a fluorescence assay (Qubit or Picogreen).
- Samples should be resuspended in nuclease free water or low EDTA TE buffer.
- The 260:280 OD ratio should be 1.8 - 2.0 and the 260:230 OD ratios >1.5.
- DNA should be intact as assessed by agarose gel or similar method.
Pluripotency Confirmation Arrays
- 10ul of intact total RNA at a concentration of 50-100 ng/ul.
Oxford Nanopore Long Read Sequencing
Contact us for further information or refer to your quote. Our Long & Linked Read Sample Submission Guide provides general information on DNA/RNA quality.
Fluidigm & Nanostring Services
Contact us for further information or refer to your quote.