The most accurate sample submission information can be found in our quotes. The information below serves only as a general guide on sample requirements by application e.g. RNA sequencing. 

General Sample Submission Criteria

  • Samples should be intact i.e. not degraded.
  • Samples should be free of contaminants: 260:280 and 260:230 ratios >1.8.
  • RNA samples must be free of genomic DNA contamination.
  • DNA samples must be free of RNA contamination.
  • DNA concentration should be measured using the Qubit or Picogreen assay as quantification by spectrophotometric methods is inaccurate.


Application Specific Sample Submission Criteria


Genome Sequencing - Short Read
 

Service  Amount Required Concentration Minimum Volume
TruSeq PCR-free

4 ug DNA

50-100 ng/ul

60 ul

Nextera DNA Flex

500ng DNA

20 ng/ul

25 ul

Nextera XT DNA

100ng DNA

5 ng/ul

20 ul

 

RNA Sequencing 
 

Service  Amount Required Concentration Minimum Volume
mRNA seq

3 ug total RNA

70-100 ng/ul

40 ul

Total RNA seq*

3 ug total RNA

120-180 ng/ul

25 ul

Total RNA seq - low input

10-20 ng total RNA

1-2 ng/ul

10 ul

Total RNA seq - rRNA depleted submission

300 ng rRNA depleted total RNA 

20-30 ng/ul

15 ul

small RNA

1 ug total RNA
(low input options available)

80-100 ng/ul

12 ul

* Species supported: human, mouse, rat and plant.  

 

Gene Expression & Transcriptome Microarrays
 

Service  Amount Required Concentration Minimum Volume
Standard assay

500 ng total RNA

50-100 ng/ul

10 ul

Low input (pico) assay

500 pg total RNA

50-100 pg/ul

10 ul

 

PacBio Long Read & Chromium 10X Linked Read Sequencing 

Contact us for further information or refer to your quote. Our Long & Linked Read Sample Submission Guide provides general information on DNA/RNA quality. 
 

Exome and Panel Sequencing 

We provide various capture options requiring different input amounts. Contact us for further information. 
 

Microbiome Sequencing

  • 15-20ul of DNA at a concentration of 5-10ng/ul.
  • Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
  • DNA of high purity (260/280, 260/230 >~1.8).
  • Samples must be amplifiable by PCR (please check this prior to submission).


ChIP Sequencing

  • 10ng of the enriched material in a total volume of 20ul.
  • Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
  • The fragment size range of the enriched material should be 200-600bp.  
     

Client Prepared Libraries 

  • Refer to page 1 of your quote for the amount for this service.
  • Library fragments up to 1kb.  
  • Custom primers must be supplied at the time of sample submission.
  • One pool per run (unless prior arrangements have been made).


Genotyping by Microarray 

  • 50ul of intact genomic DNA at a concentration of 10 ng/ul.
  • Quantification must be performed by a fluorescence assay (Qubit or Picogreen).
     

Fluidigm & Nanostring Services

Contact us for further information or refer to your quote.