The Ramaciotti Centre for Genomics provides a range of targeted next-generation sequencing services that enable the taxonomic profiling of mixed communities. These methods use primers designed to target and capture taxonomic regions, followed by next-generation sequencing. Targeted sequencing approaches to study the microbiome are often known as diversity profiling or amplicon, metabarcoding or tag sequencing.
Costs start at $30/sample for our standard microbiome sequencing services.
All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
Microbiome Sequencing Services -16S/18S/ITS
We have a range of pre-optimised gene region targets ready to go, refer to the table below. The workflow includes PCR to incorporate unique barcodes for each sample, pooling of samples in to a single library, and sequencing on an Illumina MiSeq.
|Target||Gene Region||Primers||Used By*|
|Bacteria||16S V1-V3||27f - 519r||BASE|
|16S V4||515f - 806r||EMP|
|18S V9||1391f - EukBr||BASE, EMP|
|Eukaryote - Fungi||ITS1||ITS1F-ITS2||EMP|
|Archea||A16S||A2f - 591r||BASE|
* MM - Australian Marine Microbes project, BASE - Biome of Australia Soil Environments (BASE) project, EMP - Earth Microbiome Project. BASE and Marine Microbes are Bioplatforms Australia Initiatives.
Custom Target Sequencing Services
If you would like to target genes not listed above we can offer a custom short read approach based on a two-step PCR design. For this you would perform an initial round of PCR using a pair of gene/locus specific primers which include Illumina adapter overhangs and then submit the purified product to us for sequencing. Multiple gene targets can be pooled in a sequencing run if they are size compatible.
Custom amplicons can also be sequenced using Pacbio technology. PacBio offers longer read lengths (~6kb) and provides phased sequencing for identification of strand specific base changes.
We would be happy to advise you on our custom target sequencing, please contact us.