The Ramaciotti Centre for Genomics provides a range of targeted next-generation sequencing services that enable the taxonomic profiling of mixed communities. These methods use primers designed to target and capture taxonomic regions, followed by next-generation sequencing.  Targeted sequencing approaches to study the microbiome are often known as diversity profiling or amplicon, metabarcoding or tag sequencing.

Costs start at $30/sample for our standard microbiome sequencing services. 

All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
 

Microbiome Sequencing Services -16S/18S/ITS

We have a range of pre-optimised gene region targets ready to go, refer to the table below. The workflow includes PCR to incorporate unique barcodes for each sample, pooling of samples in to a single library, and sequencing on an Illumina MiSeq. 
 

Target Gene Region Primers Used By*
Bacteria 16S V1-V3 27f - 519r BASE
  16S V3-V4 341f-805r  
  16S V4 515f - 806r EMP 
Eukaryote 18S V4 18SV4F_18SV4R MM
  18S V9 1391f - EukBr BASE, EMP
Eukaryote - Fungi ITS1 ITS1F-ITS2 EMP
  ITS2 fITS7-ITS4  
Archea A16S A2f - 591r BASE
* MM - Australian Marine Microbes project, BASE - Biome of Australia Soil Environments (BASE) project, EMP -  Earth Microbiome Project. BASE and Marine Microbes are Bioplatforms Australia Initiatives

 

Custom Target Sequencing Services

If you would like to target genes not listed above we can offer a custom short read approach based on a two-step PCR design. For this you would perform an initial round of PCR using a pair of gene/locus specific primers which include Illumina adapter overhangs and then submit the purified product to us for sequencing. Multiple gene targets can be pooled in a sequencing run if they are size compatible. 
Custom amplicons can also be sequenced using Pacbio technology. PacBio offers longer read lengths (~6kb) and provides phased sequencing for identification of strand specific base changes.  
We would be happy to advise you on our custom target sequencing, please contact us