The Ramaciotti Centre for Genomics provides a range of targeted next-generation sequencing services that enable the taxonomic profiling of mixed communities. These methods use primers designed to target and capture taxonomic regions, followed by next-generation sequencing.  Targeted sequencing approaches to study the microbiome are often known as diversity profiling or amplicon, metabarcoding or tag sequencing. The cost per sample for our standard microbiome sequencing services starts at $30 per sample. 

All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
 

Microbiome Sequencing Services

We have a range of pre-optimised gene region targets ready to go, refer to the table below. The workflow includes PCR to incorporate unique barcodes for each sample, pooling of samples in to a single library, and sequencing on an Illumina MiSeq. 
 

Target Gene Region Primers Used By*
Bacteria 16S V1-V3 27f - 519r BASE
  16S V3-V4 341f-805r  
  16S V4 515f - 806r EMP 
Eukaryote 18S V4 18SV4F_18SV4R MM
  18S V9 1391f - EukBr BASE, EMP
Eukaryote - Fungi ITS1 ITS1F-ITS2 EMP
  ITS2 fITS7-ITS4  
Archea A16S A2f - 591r BASE
* MM - Australian Marine Microbes project, BASE - Biome of Australia Soil Environments (BASE) project, EMP -  Earth Microbiome Project. BASE and Marine Microbes are Bioplatforms Australia Initiatives

 

Custom Target Sequencing Services

If you would like to target genes not listed above we can offer a custom short read approach based on a two-step PCR design. For this you would perform an initial round of PCR using a pair of gene/locus specific primers which include Illumina adapter overhangs and then submit the purified product to us for sequencing. Multiple gene targets can be pooled in a sequencing run if they are size compatible. 
Custom amplicons can also be sequenced using Pacbio technology. PacBio offers longer read lengths (~6kb) and provides phased sequencing for identification of strand specific base changes.  
We would be happy to advise you on our custom target sequencing, please contact us