The Ramaciotti Centre for Genomics provides a range of targeted next-generation sequencing services that enable the taxonomic profiling of mixed communities. These methods use primers designed to target and capture taxonomic regions, followed by next-generation sequencing.  Targeted sequencing approaches to study the microbiome are often known as diversity profiling or amplicon, metabarcoding or tag sequencing.

Costs start at $30/sample for our standard microbiome sequencing services. 

All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.

Microbiome Sequencing Services -16S/18S/ITS

We have a range of pre-optimised gene region targets ready to go, refer to the table below. The workflow includes PCR to incorporate unique barcodes for each sample, pooling of samples in to a single library, and sequencing on an Illumina MiSeq. 

Target Gene Region Primers Used By*
Bacteria 16S V1-V3 27f - 519r AM, BASE
  16S V3-V4 341f-805r  
  16S V4 515f - 806r EMP 
  16S full length 27f - 1492r ONT - rapid 16S kit 
Eukaryote 18S V4 18SV4F_18SV4R AM, MM
  18S V9 1391f - EukBr AM, BASE, EMP
Eukaryote - Fungi ITS1 ITS1F-ITS2 EMP
  ITS2 fITS7-ITS4  
Archea A16S A2f - 591r AM, BASE
* AM - Australian Microbiome project, MM - Marine Microbes project, BASE - Biome of Australia Soil Environments (BASE) project, EMP -  Earth Microbiome Project.

The AM, BASE and MM projects are Bioplatforms Australia Initiatives


Custom Target Sequencing Services

If you would like to target genes not listed above we can offer a custom short read approach based on a two-step PCR design. For this you would perform an initial round of PCR using a pair of gene/locus specific primers which include Illumina adapter overhangs and then submit the purified product to us for sequencing. Multiple gene targets can be pooled in a sequencing run if they are size compatible. Custom amplicons can also be sequenced using Oxford Nanopore long read technology. 

We would be happy to advise you on our custom target sequencing, please contact us