Hi-C is a genome-wide chromatin conformation capture protocol that uses proximity ligation. It is used to generate libraries that link genomic regions that are in close spatial proximity. The sequence data from these libraries can be used to scaffold genomic assemblies, detect structural variation, and provide 3D genome conformation information.
The Ramaciotti Centre offers a Hi-C service using the Dovetail Genomics or Phase Genomics kit. Obtaining the best results from Hi-C sequence data requires quality input material (tissue or cells) and a high-quality draft genome assembly.
Our Hi-C Guide provides information on sample input for the assay and guidance on the draft genome assembly data that is required for genome scaffolding. If you do not have a draft genome assembly we can work with you to generate one using our short and long read sequencing services.