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Sequencing | Technology

The Centre has HiSeq 2500, NextSeq 500, MiSeq and PacBio sequencing platforms.

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Specifications of our Sequencing Platforms

 HiSeq 2500NextSeq  MiSeqPacBio
Key
applications
Exomes
Genomes
Transcriptomes
Exomes
Genomes
Transcriptomes
Small genomes
Amplicons
Targeted panels
Genomes
RNA Isoform identification
Targeted sequencing
Max read number350M SE
700M PE
Mid
130M SE
260M PE
High
400M SE
800M PE
25M SE
50M PE
NA
Max output170 Gb40 Gb120 Gb 15 Gb1 Gb
Max read length2 x 250 bp2 x 150bp 2 x 150bp 2 x 300bpAverage 10 kb
(1 kb - 50 kb)
Run time40 hours15-26 hours15-26 hours5-55 hours72 hours

Useful Links

For comprehensive information on Illumina sequencing platforms you can visit the Illumina or PacBio websites. Alternatively you can contact the Centre to discuss your project.

Please visit our Frequently Asked Questions page for general information on services.

For instructions on how to submit samples please read the our Sample Submission Guide.

Illumina’s sequencing coverage calculator can help determine the number of runs required to arrive at the correct coverage for your experiment.

For further information on the most suitable kit type for your project consult Illumina’s kit selector or contact the Centre for assistance with your project design.  

 

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