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Sequencing | Technology

 

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Specifications of our Illumina Sequencing Platforms

 NovaSeq 6000HiSeq 2500NextSeq - High OutputNextSeq - Mid OutputMiSeq
Key
applications
Exome
Genome
Transcriptome
Genome
Transcriptome

Exome
Genome
Transcriptome
Genome
Transcriptome
Amplicon
Small genome
Targeted panels
Max read number - paired3.3B 350M
400M
130M
25M
Max output1000 Gb170 Gb120 Gb 40 Gb15 Gb
Max read length2 x 150 bp2 x 250 bp2 x 150bp 2 x 150bp 2 x 300bp
Run time19-40 hours40 hours15-26 hours15-26 hours5-55 hours

Specifications of our PacBio Sequencing Platforms

 SequelRSII
Key
applications
Genomes
RNA Isoform Identification
Amplicon
Targeted
Genomes
RNA Isoform Identification
Amplicon
Targeted
Max read number370,000
55,000-65,000
Max output7 Gb1 Gb
Max read lengthAverage 10kb
(1kb - 50kb)
Average 10kb
(1kb - 50kb)

Useful Links

For more detailed  information on our sequencing platforms you can visit either the Illumina or PacBio websites. Alternatively you can contact the Centre to discuss your project.

Please visit our Frequently Asked Questions page for general information on services.

For instructions on how to submit samples please read the our Sample Submission Guide.

Illumina’s sequencing coverage calculator can help determine the number of runs required to arrive at the correct coverage for your experiment.

For further information on the most suitable kit type for your project consult Illumina’s kit selector or contact the Centre for assistance with your project design.  

 

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