Targeted genomic sequencing focuses on specific areas of interest and reduces sequencing costs. There is a wide range of products available, for a list of the most commonly used refer to the table below.
Please contact us for us for a quote or further information on targeted sequencing.
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Targeted Sequencing Products
|Illumina||TruSeq & TruSight Panels||Human||Disease specific and custom panels available. Low input.|
|Illumina||TruSeq Exome or Nextera Rapid Capture||Human||Custom and expanded (miRNA and UTR region) available.|
|Illumina||TruSeq Targeted RNA Expression||Human||Low input, from 50ng RNA.|
|Agilent||Haloplex||Human||Low input, from 50ng DNA.|
|Agilent||SureSelect All Exon||Human and mouse. Other species available through custom design.||Custom and expanded (UTR region) available. Pre and post capture pooling options.|
|Fluidigm||Access Array||Any species||Refer to text below|
The Fluidigm Access Array system is a target enrichment platform designed for resequencing of selected regions in large numbers of samples. The Access Array system works by assembling PCR reactions for 48 samples against 48 primer sets. Up to 48 primer pairs can be used in singleplex format, or up to 480 primer pairs can be used in the multiplex format. The system is open platform allowing you to use primers from any many sources, whether they are user-designed or ordered through Fluidigm. Please refer to our primer design page for further information.
Predesigned Primer Panels
Fluidigm have predesigned primer panels that have been optimised to work with the Access Array system. Panels are available for:
- BRAC1 – BRAC2 – TP53
The Ramaciotti Centre has established a pipeline to carry your samples from the Access Array system through to the Illumina Sequencing platforms. Please contact us for further information or assistance with experimental design.