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Sequencing | Sample Submission

To submit a project and ensure its smooth and speedy completion, please follow these 6 steps:

1. Obtain a quote:

  • Email us your project details and we will prepare a quote. 
  • An indication of sample number and the species you are working on helps us with costing. 

2. Accept the quote: 

  • Email us a copy of your purchase order. 
  • Paying by credit card? Request an invoice and pay in full before sample submission. 
  • From UNSW? Provide your UNSW project code at the time of online submission.

3. Check that you have enough sample:

  • Check that your samples meet the general sample requirements listed below.

– Samples should be intact and not degraded.
– Samples should be clean, 260:280 and 260:230 ratios >1.8.
– RNA samples must be free of genomic DNA contamination.
– DNA samples must be free of RNA contamination.

  • Refer to page 1 of your quote for the amount, concentration and volume required for the service or follow the links below.
  • Refer to page 2 of your quote for specific submission instructions e.g. resuspension buffer, plate layout etc. 
  • Issues with quantity or quality? Please contact us for advice. 

Application Specific Sample Requirements:

4. Submit your order online:

  • New customer?  Sign up for an account and read our guide to online sample submission.
  • Returning customer? Login to your account. 
  • To avoid upload errors download a new copy of the submission form each time you submit.

5. Prepare your samples:

  • Refer to page 2 of your quote for project specific submission instructions. 
  • Label your tubes/plates with the unique ID issued during online submission (step 4 above). 
  • Projects with <10 samples can be submitted in 1.5 ml tubes, unless otherwise specified. 
  • Do not use screw cap or strip tubes, or seal tubes with parafilm, foil or tape. 
  • Projects with >10 samples must be submitted in 96-well PCR plates. 
  • Make sure that plates are well sealed and that the seal adheres at the shipping temp e.g. -80C.
  • If the project requires multiple runs/plates, randomise your samples. 

6. Packing and shipping:

  • Our recommended couriers are World Courier and PDP Couriers. 
  • Place tubes/plates in a plastic bag and wrap in bubble wrap. 
  • If shipping on dry ice use granular dry ice not dry ice blocks and include enough dry ice to last for two days. 
  • Include a printed copy of the electronic submission form with the package.
  • Notify us by email when your samples have been shipped and provide us with an approximate delivery date. 
  • If you are shipping from overseas contact us to obtain a copy of our import permit. 

Shipping Address
Ramaciotti Centre for Genomics
Upper Campus Store, Room LG018
E26, Bioscience South
LG018 Loading Dock,
Via Gate 11 Botany Street,
UNSW Sydney NSW 2052
Australia
Tel: (02) 9385 1241
Hours: Monday to Friday, 8.00am – 4.00pm

In Person Drop off
Ramaciotti Centre for Genomics
Level 2, Biosciences South Building (E26)
Botany Street
UNSW Sydney, NSW  2052
Tel: (02) 9385 1241
Hours: Monday to Friday, 8.30am – 5.00pm
Please check that there will be someone available to accept them by calling (02) 9385 1241.

 

 

Application Specific Sample Submission Criteria

Exome & Genome Sequencing

  • DNA must be double-stranded and not degraded as assessed by 0.8% agarose gel. 
  • DNA concentration should be measured using the Qubit or Picogreen assay. Quantification by NanoDrop is inaccurate. 

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SERVICEAMOUNT REQUIREDCONCENTRATIONMINIMUM VOLUME
DNA Seq (PCR-Free)4 ug 50-100 ng/ul60 ul
Nextera DNA Flex400 ng20 ng/ul20 ul
Nextera XT DNA100 ng5 ng/ul20 ul
ExomeContact usContact usContact us

 

RNA sequencing 

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APPLICATIONAMOUNT REQUIREDCONCENTRATIONVOLUME
mRNA Seq 3 ug total RNA70-100 ng/ul40-50 ul
Standard Total RNA Seq RNA - h/m/r & plant3 ug total RNA100-150 ng/ul25-30 ul
Low Input Total RNA Seq RNA - h/m/r 10-20 ng total RNA1-2 ng/ul 10-15 ul
Total RNA Seq - rRNA depleted submission300 ng rRNA depleted RNA15-20 ng/ul12-15 ul
small RNA1 ug total RNA*80-100 ng/ul12-15 ul

*Low input options are available. Please contact us.

ChIP Sequencing

  • 10ng of the enriched material in a total volume of 20ul.
  • Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
  • The fragment size range of the enriched material should be 200-600bp.  

Amplicon/Microbial Community Profiling

  • 15-20ul of DNA at a concentration of 5-10ng/ul.
  • Quantification should be performed by a fluorescence assay (Qubit or Picogreen). 
  • DNA of high purity (260/280, 260/230 >~1.8).  
  • Samples must be amplifiable by PCR. Please check this prior to submission. 

Libraries (Client Supplied)

  • Refer to page 1 of your quote for the amount for this service.
  • Library fragments up to 1kb.  
  • Custom primers must be supplied at the time of sample submission.
  • One pool per run (unless prior arrangements have been made).

Long & Linked Read Sequencing

Please download our Long & Linked Read Sample Submission Guide for further information on DNA/RNA quality and concentration. 

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