The Centre is experienced in a wide variety of protocols allowing us to tailor our service to your research project. We provide excellent project support and deliver high quality data at competitive prices.
To ensure quality we:
- perform extensive QC checks on your RNA samples
- minimise PCR cycles, thereby reducing the number of duplicate reads in your data
- only use stranded kits to increase mapping accuracy
If required we can provide a rRNA removal service on:
- Plant Leaf/Seed/Root
Further information on rRNA kit species compatibility can be found on Illumina’s website.
The RNA sequencing services that we offer are summarised in the table below. Please contact the Centre for further information or a quote.
Flip device horizontal to see full table
|Total RNA||Poly A pull down. |
Coding only. Stranded.
|All except bacterial & viral||Illumina|
|Total RNA Seq Stranded - depleted submission||rRNA depleted RNA||Coding and non-coding RNA.|
|Total RNA Seq|
|Total RNA||Ribosomal depletion. |
Coding and non-coding RNA. Stranded.
|Refer to Illumina's website, see above.||Illumina|
|Low Input RNA Seq||Total RNA||Coding and non-coding RNA. Stranded.||All||Illumina|
|Small RNA||Total RNA||small RNA up to 36nt||All||Illumina|
|RNA Capture||Total RNA||Targeted RNA enrichment enables sequencing of regions of interest.||Refer to Agilent website||Illumina|
|Iso-Seq||cDNA||De novo transcriptome and isoform analysis.||All||PacBio|
For instructions on how to submit samples please read the NGS Sample Submission Guide.
For general information on our NGS services please visit our FAQ page.
Further information on sequencing platforms can be found on our technology page.