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Sequencing | RNA Sequencing

RNA Sequencing Services

The Ramaciotti Centre for Genomics has been providing next generation sequencing sequencing services to the research community since 2009. We have experience using a wide variety of RNA sequencing protocols allowing us to tailor our services to your research project. The Centre provides excellent project support, assisting with design and approach and delivers high quality data at competitive prices.

To ensure quality we:

  • perform extensive QC checks on your RNA samples 
  • minimise PCR cycles, thereby reducing the number of duplicate reads in your data
  • only use stranded kits to increase mapping accuracy

If required we can provide a rRNA removal service for:

  • Human/Mouse/Rat
  • Bacteria
  • Plant Leaf/Seed/Root
  • Yeast

Further information on rRNA kit species compatibility can be found on Illumina’s website.  

The RNA sequencing services that we offer are summarised in the table below. Please contact the Centre for further information or a quote.

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mRNA Seq
Total RNAPoly A pull down -
coding only - stranded
All except bacterial & viralIllumina
Total RNA Seq
Total RNArRNA depletion -
coding & non-coding RNA - stranded
Refer to the Illumina websiteIllumina
Total RNA Seq (rRNA depleted sample)rRNA depleted RNA Coding & non-coding RNA - strandedAll Illumina
Low Input RNA SeqTotal RNACoding & non-coding RNA - strandedAll Illumina
Small RNATotal RNAsmall RNA up to 36ntAllIllumina
RNA CaptureTotal RNAEnrichment of regions of interestRefer to the Agilent websiteIllumina
Iso-SeqcDNADe novo transcriptome and isoform analysisAllPacBio

Useful Links

For instructions on how to submit samples for RNA sequencing please read the NGS Sample Submission Guide.

For general information on our NGS services please visit our FAQ page.

Further information on sequencing platforms can be found on our technology page.

  • Services Offered

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