RNA Sequencing Services
The Ramaciotti Centre for Genomics has been providing next generation sequencing sequencing services to the research community since 2009. We have experience using a wide variety of RNA sequencing protocols allowing us to tailor our services to your research project. The Centre provides excellent project support, assisting with design and approach and delivers high quality data at competitive prices.
To ensure quality we:
- perform extensive QC checks on your RNA samples
- minimise PCR cycles, thereby reducing the number of duplicate reads in your data
- only use stranded kits to increase mapping accuracy
If required we can provide a rRNA removal service for:
- Human/Mouse/Rat
- Bacteria
- Plant Leaf/Seed/Root
- Yeast
Further information on rRNA kit species compatibility can be found on Illumina’s website.
The RNA sequencing services that we offer are summarised in the table below. Please contact the Centre for further information or a quote.
Flip device horizontal to see full table
| SERVICE | INPUT | DESCRIPTION | SPECIES | SEQUENCING TECHNOLOGY |
|---|---|---|---|---|
| mRNA Seq | Total RNA | Poly A pull down - coding only - stranded | All except bacterial & viral | Illumina |
| Total RNA Seq | Total RNA | rRNA depletion - coding & non-coding RNA - stranded | Refer to the Illumina website | Illumina |
| Total RNA Seq (rRNA depleted sample) | rRNA depleted RNA | Coding & non-coding RNA - stranded | All | Illumina |
| Low Input RNA Seq | Total RNA | Coding & non-coding RNA - stranded | All | Illumina |
| Small RNA | Total RNA | small RNA up to 36nt | All | Illumina |
| RNA Capture | Total RNA | Enrichment of regions of interest | Refer to the Agilent website | Illumina |
| Iso-Seq | cDNA | De novo transcriptome and isoform analysis | All | PacBio |
Useful Links
For instructions on how to submit samples for RNA sequencing please read the NGS Sample Submission Guide.
For general information on our NGS services please visit our FAQ page.
Further information on sequencing platforms can be found on our technology page.
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