How much does it cost per sample?
We do not have pricing on our website as the cost per sample is very dependent on sample number and the application. Please contact us providing as much information as possible on your project and we will provide you with a quote.
How much sample do I need and how do I submit my samples?
Please refer to your quote of to our How to Submit Samples page. An online sample submission form must be completed before sending your samples. There is a direct link to our online submission portal on our home page.
How long will it take to get my data?
Turnaround times are dependent on project size and whether we have the required reagents in stock. We advise of the current turnaround times on our quotes. Be assured that we try our best to get data to you as fast we can, we don’t like to keep anyone waiting.
What bioinformatics support is available at the Centre?
Please visit our bioinformatics page if you need bioinformatics support for your downstream analysis.
What does the Ramaciotti Centre do to ensure quality?
The Ramaciotti Centre for Genomics is committed to providing competitive, high quality genomics services. We do this through:
– Consistently providing quality analytical services to our clients through data that meets or exceeds manufacturer’s specifications.
– Ensuring that all personnel are competent and qualified for the tasks they perform.
– Developing and applying a quality management system according to the ISO/IEC17025 Standard.
All samples submitted to the Ramaciotti Centre must first pass initial quality control (QC) checks. A threshold for these checks has been put in place to ensure you receive data of the highest quality. We know from experience that samples below these thresholds may not perform well so advise clients to where possible meet or exceed them. Clients are notified if their samples do not pass QC and are advised of the next steps and possible solutions.
If a sample fails initial quality control clients can resubmit samples or withdraw their submission. If the client chooses to resubmit their samples they must do so within a 4 week period. Sample submission after the 4 week period has elapsed will be considered as a new submission. The decision on whether to process a sample remains with the client. In the case where a sample that does not pass our QC standards is processed, the Ramaciotti Centre will guarantee the outcome and the client must accept responsibility. If the client withdraws their sample submission they must pay for any consumables ordered on their behalf. A $15 fee per failed sample is charged to cover the cost of consumables and labour.
What is the best method to use for extracting RNA?
We do not recommend a particular method. If you have an established method that yields good quality, clean RNA please continue to use this. Please note that extraction methods that use organic solvents such as TRIzol Reagent may result in inaccurate quantification, due to the carry over of organic solvents. These solvents may compress the 260/230 ratio and often result in an overestimation of the amount of total RNA. A final column clean up method is recommended before submission. For further information please read our RNA purification guide.
What is the best method to use for extracting small RNA?
For small RNA arrays and sequencing we require total RNA input so there is no need to isolate small RNAs. As with total RNA we do not recommend a particular method. If you are using a commercial kit ensure that it retains small RNAs and follow the total RNA isolation protocol. Do not use the size fractionation or small RNA enrichment protocol. Please ensure that you use the same extraction protocol for all of the samples being analysed as different total RNA extraction methods may result in different miRNA profiles.
Should I include replicates in my RNA sequencing or gene expression microarray experiment?
RNAseq or gene expression experiments should be treated like any other experiment in which you would as a matter of course include biological replicates. Consulting with bioinformatician on the number of replicates required for your experimental design is advisable as insufficient biological replicates will lead to an increase in the false discovery rate. There are a number of technical publications on RNA sequencing that may be of help. Schurch et al RNA (2016) on the number of replicates need for RNA sequencing and Corley et al BMC Genomics (2017) on the use of stranded protocols and single read versus paired end methods.
Where can I find general information on next generation sequencing?
Read Illumina’s sequencing introduction to find out more about short read sequencing or visit the PacBio website to learn more about long read sequencing. The Centre also runs a series of NGS focused workshops and seminars throughout the year. To learn more about these sign up to our mailing list.
Which sequencer should I use for my project?
Please visit our technology page for further information on the different technologies we have or contact us for project planning assistance. Illumina’s sequencing coverage calculator can help determine the number of runs required to arrive at the correct coverage for your experiment.