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Fluidigm | SNP Genotyping

The Ramaciotti Centre provides a cost effective, high throughput single nucleotide polymorphism (SNP) genotyping service using the Fluidigm BioMark HD™. Fluidigm offers the ideal solution for studies requiring ultra-low cost and high-sample throughput for low- to midplex SNP genotyping.

Services

The availability of different plate formats makes the Fluidigm BioMark HD™ suitable for small to large scale SNP genotyping projects, refer to the table below.  Please contact us for a quote or for further information on these services.

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PLATE FORMATS96.96192.2448.48
No. of Samples9619248
No. of Assays962448
Reactions per run9,2164,6082,304

Fluidigm Chip Pipetting Map

Fluidigm_IFC_layout

Fluidigm Chip Pipetting Map of a 48.48 Integrated Fluidics Circuit (IFC): 48 samples run against 48 assays, 2,304 reactions are processed independently and in parallel in nano reaction chambers (not multiplexed).

Compatible Assay Types

Fluidigm IFCs are compatible with two different assay types. Please refer to the table below for further information.

ASSAYSUPPLIERDESIGNCHEMISTRY
SNPType™FluidigmPre-designed
Custom design
FAM™ or Hex™
TaqMan®Life TechnologiesPre-designed
Custom design
FAM™ or VIC®

IMPORTANT: Customers must validate their assays (primers) before submission. As the Fluidigm BioMark HD is different to other PCR systems it is recommended that a trial run is performed prior to scaling up. The Centre will not guarantee results if assays are not validated on the Fluidigm system. Please contact us for validation protocols and assistance with experimental design.

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