The Ramaciotti Centre provides a cost effective, high throughput single nucleotide polymorphism (SNP) genotyping service using the Fluidigm BioMark HD™. Fluidigm offers the ideal solution for studies requiring ultra-low cost and high-sample throughput for low- to midplex SNP genotyping.
The availability of different plate formats makes the Fluidigm BioMark HD™ suitable for small to large scale SNP genotyping projects, refer to the table below. Please contact us for a quote or for further information on these services.
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|No. of Samples||96||192||48|
|No. of Assays||96||24||48|
|Reactions per run||9,216||4,608||2,304|
Fluidigm Chip Pipetting Map
Fluidigm Chip Pipetting Map of a 48.48 Integrated Fluidics Circuit (IFC): 48 samples run against 48 assays, 2,304 reactions are processed independently and in parallel in nano reaction chambers (not multiplexed).
Compatible Assay Types
Fluidigm IFCs are compatible with two different assay types. Please refer to the table below for further information.
|FAM™ or Hex™|
|FAM™ or VIC®|
IMPORTANT: Customers must validate their assays (primers) before submission. As the Fluidigm BioMark HD is different to other PCR systems it is recommended that a trial run is performed prior to scaling up. The Centre will not guarantee results if assays are not validated on the Fluidigm system. Please contact us for validation protocols and assistance with experimental design.