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Fluidigm | Gene Expression – qPCR

The Ramaciotti Centre provides a cost effective quantitative real-time PCR (qPCR) service using the Fluidigm BioMark HD™. Our Fluidigm qPCR service is well suited for validation studies or investigations that do not require a whole genome approach such as microarray or RNA seq.

qPCR experiments are often limited by technology that only allows the study of one or two genes at a time in small numbers of samples. The high throughput of the BioMark HD™ allows the inclusion of numerous controls and biological replicates in a single run with minimal pipetting steps, thereby increasing the accuracy of your data.

Services

The availability of different plate formats makes the Fluidigm BioMark HD™ suitable for small to large scale qPCR experiments, refer to the table below. Please contact us for a quote or for further information on these services.

Flip device horizontal to see full table

PLATE FORMATS96.96192.2448.48
No. of Samples9619248
No. of Assays962448
Reactions per run9,2164,6082,304

Fluidigm Chip Pipetting Map

Fluidigm_IFC_layout

Fluidigm Chip Pipetting Map of a 48.48 Integrated Fluidics Circuit (IFC): 48 samples run against 48 assays, 2,304 reactions are processed independently and in parallel in nano reaction chambers (not multiplexed).

Compatible Assay Types

Fluidigm IFCs are compatible with a number of different assay types. Please refer to the table below for more information.

ASSAYSUPPLIERDESIGNCHEMISTRY
DELTAgene™FluidigmPre-designed
Custom design
EVAGreen®
(similar to SYBR® green)
TaqMan®Life TechnologiesPre-designed
Custom design
FAM™ or VIC®
CustomerAnyYour own designEVAGreen®
(similar to SYBR® green)

IMPORTANT: Customers must validate their assays (primers) before submission. As the Fluidigm BioMark HD is different to other qPCR systems it is recommended that a trial run is performed prior to scaling up. The Centre will not guarantee results if assays are not validated on the Fluidigm system. Please contact us for validation protocols and assistance with experimental design.

 

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