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Array | Sample Submission Guide

To submit samples please follow these five steps:

1. Online sample submission:

Before sending your samples please electronically submit them to the Centre using our Customer Sample Submission Portal.
–  New customers are required to sign up for a user ID.  Applications will be reviewed and approved by the Centre before being activated. For further instructions please refer to our creating a User ID guide.
– Follow the submission instructions carefully or the Excel spreadsheet will fail to upload.  Please refer to the guide to submitting samples for detailed instructions. 
–  The system will issue a unique ID for each sample it is important that you label tubes and plates with this ID. Samples that are not labelled with the unique LIMS ID will not be processed.

2. Assay Specific Sample Criteria:

Please consult the tables below for the amount, concentration and volume of nucleic acid required for each assay. If you cannot provide the amount of material stated below please contact us.

RNA Analysis by Micorarray: Sample Requirements

PLATFORMMICROARRAY TYPESAMPLE REQUIREDMINIMUM AMOUNT REQUIREDREQUIRED CONCENTRATIONMINIMUM VOLUME
AffymetrixRNA analysisTotal RNA500 ng*50-100 ng/µl10 µl
miRNA analysisTotal RNA500 ng50-100 ng/µl10 µl
AgilentRNA analysisTotal RNA500 ng*50-100 ng/µl10 µl
miRNA analysisTotal RNA500 ng50-100 ng/µl10 µl

*If submitting samples for a low input assay please contact the Centre.

DNA Analysis by Microarray: Sample Requirements

PLATFORMMICROARRAY TYPESAMPLE REQUIREDMINIMUM AMOUNT REQUIREDREQUIRED CONCENTRATIONMINIMUM VOLUME
AffymetrixAxiom GenotypingGenomic DNA750 ng15 ng/µl50 µl
MicrobiomeGenomic DNA62.5 ng2.5 ng/µl25 µl
CytoScanGenomic DNA500 ng50 ng/µl10 µl
OncoScan Genomic DNA108ng12 ng/µl9 µl
AgilentCNV
CGH
SNP
Genomic DNAPlease contact us.Please contact us.Please contact us.

 

IMPORTANT:
If we receive samples that do not comply with sample submission guidelines they will not be processed. You can either:
i) Resubmit samples. If samples are provided in plates the entire plate must be resubmitted. We will not cherry pick samples.
ii) Arrange for samples to be returned. The client must arrange courier collection and liaise with the Centre on the scheduling of this collection. The Centre is not responsible for any courier fees incurred for sample return.
Resubmitted samples must be labelled with a new unique LIMS ID. Samples will not be accepted or processed if they are labelled with a previous submissions project ID.

Samples that fail QC and do not proceed to array will be attract a $15 QC charge to cover costs.  For further information on the quality control procedures we use, please visit our Sample Quality Control page. If you wish to know more about preparation of RNA for microarray please download our RNA purification guide.

3. General Sample Submission Criteria:

Please ensure that your samples meet the following quality criteria:

  • Samples must be intact and not degraded, as assessed by Bioanalyzer, TapeStation or LabChip GX.
  • Samples must be clean, 260:280 and 260:230 ratios >1.8. Lower 260:230 ratios are acceptable if a column purification method was used.
  • RNA samples must be free of genomic DNA contamination.
  • DNA samples must be free of RNA contamination.
  • Samples must be resuspended in nuclease free water and not TE.
  • Samples must be at the concentration specified in the tables above.
  • Samples must be supplied at the correct minimum volume, refer to the tables above.

Preparing your samples for shipping:

  • Ensure that your samples are randomised and in the correct order for processing in batches prior to shipping. The Centre will not randomise or reorder samples.
  • Projects with sample numbers <24 can be submitted in 1.5 ml microfuge tubes (non-screw cap, no parafilm or tape). Do not use strip tubes. Please consult with the Centre if you have a larger project (n>24) as submission is preferred in 96-well PCR plates. Large submissions in tubes may attract a handling fee.
  • Tubes and plates must be clearly labeled with the corresponding LIMS ID for each sample, refer to point 1 above.
  • Ensure plates are well sealed. Freezing plates before packing and shipping prevents loss of material and ensures that plate seals remains adherent at low temperatures.
  • The Centre can supply plates and seals on request.
  • The Centre accepts no responsibility for poorly sealed plates.

 

4. Payment:

– Please email a copy of your purchase order to the Centre. Please ensure that your provide your purchasing officer with the Centre’s email address.
–  Purchase orders are generated by the purchasing department of your institution and authorise the transaction.
– If you intend to pay by credit card please email the Centre the following details: billing name, address, email and phone number.
– If you are internal to UNSW email us your project codes (Dept ID, Project ID, Fund Code).

IMPORTANT:
 – Failure to provide payment information will lead to a delay in processing your samples.

 

5. Ship your samples:

On completion of the above samples can be shipped to the Centre.

Courier Delivery Address
Ramaciotti Centre for Genomics
Faculty of Science
Upper Campus Store, Room LG018
E26, Bioscience South
LG018 Loading Dock,
Via Gate 11 Botany Street,
UNSW Sydney NSW 2052
Australia
Tel: (02) 9385 1241
Hours: Monday to Friday, 8.00am – 4.00pm

In Person Drop off
The Ramaciotti Centre
Level 2, Biosciences South Building (E26)
Botany Street
UNSW Sydney, NSW  2052
Tel: (02) 9385 1241
Hours: Monday to Friday, 8.30am – 5.00pm
Please check that there will be someone available to accept them by calling (02) 9385 1241.

 

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